Construct:Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. Plasmid construction To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA extraction and RT-PCR RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Plasmid Preparation:Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. Plasmid construction To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA extraction and RT-PCR RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Generated:Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. Plasmid construction To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA extraction and RT-PCR RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Polymerase Chain Reaction:Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. Plasmid construction To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA extraction and RT-PCR RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Clone Assay:Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
Article Title: Gastrin inhibits a novel, pathological colon cancer signaling pathway involving EGR1, AE2 and P-ERK
Article Snippet: .. Plasmid construction To construct pEGFP-C1-AE2, a 3.7 kb EcoR I-Spe I fragment from PXT7-AE2a [ 12 ], was inserted in-frame into pEGFP-C1 plasmid with DNA polymerase I (Klenow) fragment (M0210, New England Biolabs). pGL3-AE2, a human AE2 promoter reporter construct (−769/−64 relative to the initiator codon ATG) was generated by PCR and cloned into the pGL3-Basic vector (Promega, Madison, WI, USA). pEGFP-P16 and pSIREN-P16 plasmids were previously described [ 13 ]. .. RNA extraction and RT-PCR RNA was extracted from fresh colonic cancer tissue and fresh adjacent tissue with TRIZOL (15596-026, Invitrogen) following the manufacturer’s instructions.
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